2008 N W Schaad (Cultivation and Sequencing of HLB)

Cultivation and Rapid Detection of the Causal Agent of Huanglongbing Disease of Citrus

Huanglongbing (HLB), an insect-transmitted disease of citrus, also known as citrus greening, results in a green blotchy mottle on foliage, small, lopsided fruit with tree decline and eventual tree death. The causal organism has been identified as a bacterium, however no organism had been previously cultured. The protocol for identifying disease agents is to follow Koch’s postulates: isolate the pathogen from the diseased host and grow the pathogen in culture, use the culture to infect a healthy host plant and observe typical symptoms of the disease, and re-isolate the pathogen from symptomatic tissue and grow in culture.